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dc.contributor.authorAltuntaş, Aynur
dc.contributor.authorAkyol, Sümeyya
dc.contributor.authorAdam, Bahattin
dc.contributor.authorÇakmak, Özlem
dc.contributor.authorUğurcu, Veli
dc.contributor.authorErden, Gönül
dc.contributor.authorYükselten, Yunus
dc.contributor.authorDemircan, Kadir
dc.date.accessioned30.04.201910:49:13
dc.date.accessioned2019-07-13T16:31:37Z
dc.date.available30.04.201910:49:13
dc.date.available2019-07-13T16:31:37Z
dc.date.issued2015
dc.identifier.issn2148-5046
dc.identifier.urihttps://trdizin.gov.tr/publication/paper/detail/TVRjek9UY3lNZz09
dc.identifier.urihttps://hdl.handle.net/20.500.12438/1708
dc.description.abstractObjectives: In this study, we aim to investigate the association between articular damage in diabetes and a disintegrin-like and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) at gene expression and protein levels. Materials and methods: OUMS-27 human chondrosarcoma cells were used to investigate how ADAMTS12 levels changed in vitro condition in presence and absence of insulin. The study included three groups of cells treated with 10 μg/mL of insulin, and a control group. Cells were incubated with insulin in medium for one day, three days, and seven days. The effects of insulin on ADAMTS12 were investigated at both gene expression and protein levels. The relationships between the variables were tested by Mann-Whitney U test. Results: ADAMTS12 expression was significantly lower in the groups treated with insulin medium for one day and seven day periods (p=0.008 and p=0.008, respectively) compared to the control group. No significant difference was detected in the expression level between the groups kept in insulin medium for three days and the control group (p=0.55). In addition, protein amounts of the groups exposed to insulin medium for one, three, and seven day periods were lower. Conclusion: Insulin reduces the amount of ADAMTS12 which causes delayed recovery of cartilage tissue in the OUMS-27 cell lines utilized in our study for their chondrocytic properties. This reduction due to insulin treatment may contribute to recovery of cartilage tissue.en_US
dc.description.abstractObjectives: In this study, we aim to investigate the association between articular damage in diabetes and a disintegrin-like and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) at gene expression and protein levels. Materials and methods: OUMS-27 human chondrosarcoma cells were used to investigate how ADAMTS12 levels changed in vitro condition in presence and absence of insulin. The study included three groups of cells treated with 10 μg/mL of insulin, and a control group. Cells were incubated with insulin in medium for one day, three days, and seven days. The effects of insulin on ADAMTS12 were investigated at both gene expression and protein levels. The relationships between the variables were tested by Mann-Whitney U test. Results: ADAMTS12 expression was significantly lower in the groups treated with insulin medium for one day and seven day periods (p=0.008 and p=0.008, respectively) compared to the control group. No significant difference was detected in the expression level between the groups kept in insulin medium for three days and the control group (p=0.55). In addition, protein amounts of the groups exposed to insulin medium for one, three, and seven day periods were lower. Conclusion: Insulin reduces the amount of ADAMTS12 which causes delayed recovery of cartilage tissue in the OUMS-27 cell lines utilized in our study for their chondrocytic properties. This reduction due to insulin treatment may contribute to recovery of cartilage tissue.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRomatolojien_US
dc.titleADAMTS12 Depletion by Insulin in OUMS-27 Human Chondrosarcoma Cellsen_US
dc.typearticleen_US
dcterms.subjectADAMTS12en_US
dcterms.subjectCartilage Tissueen_US
dcterms.subjectİnsulinen_US
dcterms.subjectOUMS-27en_US
dc.relation.journalArchives of Rheumatologyen_US
dc.departmentTıp Fakültesien_US
dc.identifier.volume30en_US
dc.identifier.issue2en_US
dc.identifier.startpage96en_US
dc.identifier.endpage103en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US]


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