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dc.contributor.authorGenişel, Mucip
dc.contributor.authorTürk, Hülya
dc.contributor.authorErdal, Serkan
dc.contributor.authorGenç, Ebru
dc.contributor.authorTerzi, İrfan
dc.contributor.authorDemir, Yavuz
dc.date.accessioned30.04.201910:49:13
dc.date.accessioned2019-07-13T16:31:37Z
dc.date.available30.04.201910:49:13
dc.date.available2019-07-13T16:31:37Z
dc.date.issued2015
dc.identifier.issn1300-008x
dc.identifier.urihttps://trdizin.gov.tr/publication/paper/detail/TWpFek9UUTRPQT09
dc.identifier.urihttps://hdl.handle.net/20.500.12438/1710
dc.description.abstractIn the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 µM) and lead (1.75 mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase (but not catalase), as well as an increase in the level of antioxidant compounds such as ascorbic acid and glutathione, this was insufficient to ameliorate the lead-induced oxidative injury or the superoxide anion, hydrogen peroxide, and malondialdehyde levels. However, β-estradiol was able to reduce the lead-induced oxidative damage and improved the antioxidant system. Similarly, β-estradiol reduced lead-induced α-amylase activity. The effects of lead toxicity on genetic material were also determined using the randomly amplified polymorphic DNA technique. While lead led to DNA damage in wheat seedlings, β-estradiol significantly mitigated this damage. Our element analysis results show that β-estradiol did not prevent lead uptake by roots, even it did stimulate the accumulation there. Taken together, our data demonstrate for the first time that β-estradiol-induced lead tolerance is associated with many biochemical and molecular mechanisms, including the antioxidant system, detoxification of reactive oxygen species, modulation of uptake and accumulation of lead, and protection of genetic material.en_US
dc.description.abstractIn the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 µM) and lead (1.75 mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase (but not catalase), as well as an increase in the level of antioxidant compounds such as ascorbic acid and glutathione, this was insufficient to ameliorate the lead-induced oxidative injury or the superoxide anion, hydrogen peroxide, and malondialdehyde levels. However, β-estradiol was able to reduce the lead-induced oxidative damage and improved the antioxidant system. Similarly, β-estradiol reduced lead-induced α-amylase activity. The effects of lead toxicity on genetic material were also determined using the randomly amplified polymorphic DNA technique. While lead led to DNA damage in wheat seedlings, β-estradiol significantly mitigated this damage. Our element analysis results show that β-estradiol did not prevent lead uptake by roots, even it did stimulate the accumulation there. Taken together, our data demonstrate for the first time that β-estradiol-induced lead tolerance is associated with many biochemical and molecular mechanisms, including the antioxidant system, detoxification of reactive oxygen species, modulation of uptake and accumulation of lead, and protection of genetic material.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiyolojien_US
dc.subjectWheaten_us
dc.subjectLead Stressen_us
dc.subjectOxidative Stressen_us
dc.subjectAntioxidant Enzymesen_us
dc.subjectDNA Mutationsen_us
dc.titleAmeliorative role of β-estradiol against lead-induced oxidative stress and genotoxic damage in germinating wheat seedlingsen_US
dc.typeotheren_US
dc.relation.journalTurkish Journal of Botanyen_US
dc.departmentEğitim Fakültesien_US
dc.identifier.volume39en_US
dc.identifier.issue6en_US
dc.identifier.startpage1051en_US
dc.identifier.endpage1059en_US
dc.relation.publicationcategoryDiğeren_US]


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